発表論文, 総説・著書など (Published papers, Books and Reviews)


Ayako Hosoda☨, Issei Nakazato☨, Miki Okuno, Takehiko Itoh, Hideki Takanashi, Nobuhiro Tsutsumi, Shin-ichi Arimura*  TALE-based C-to-T base editor for multiple homologous genes with flexible precision Plant Biotechnology, 


☨ co-first authors,

Noémie D, Clément B, Philippe A, Delphine C, Dennis B, Taillefer Clémence G, Thomas N, Anthony R, Matthieu S, Joseph T, Daniel V, Christine C, Shin-Ichi A, Markus S, Françoise B. (2024) The mitochondrial orf117Sha gene desynchronizes pollen development and causes pollen abortion in the Arabidopsis Sha CMS  J Exp Bot,  https://doi.org/10.1093/jxb/erae214 

Toda Y, Sasaki G, Ohmori Y, Yamasaki Y, Takahashi H, Takanashi H, Tsuda M, Kajiya-Kanegae H, Tsujimoto H, Kaga A, Hirai M, Nakazono M, Fujiwara T, and Iwata H (2024) Reaction norm for genomic prediction of plant growth: modeling drought stress response in soybean. Theor Appl Genet, 137: 77.

Xu F, Su T, Zhang X, Qiu L, Yang X, Koizuka N, Arimura S, Hu Z, Zhang M, and Yang J (2024) Editing of ORF138 restores fertility of Ogura cytoplasmic male sterile broccoli via mitoTALENs. Plant Biotech J, https://doi.org/10.1111/pbi.14268

Dehaene N, Boussardon C, Andrey P, Charif D, Brandt D, Gilouppe TC, Nietzel T, Ricou A, Simon M, Tran J, Vezon D, Camilleri C, Arimura S, Schwarzländer M, and Budar F (2024) The unusual orf117Sha locus causes desynchronization of pollen development in the Arabidopsis gametophytic Sha CMS. BioRxiv, https://doi.org/10.1101/2024.01.17.575984

Nicolia A, Scotti N, D’Agostino N, Festa G, Sannino L, Aufiero G, Arimura S, and Cardi T (2024) Mitochondrial DNA editing in potato through mitoTALEN and mitoTALECD: molecular characterization and stability of editing events. Plant Methods, 20: 4. https://doi.org/10.1186/s13007-023-01124-9

Our international (Japan & Germany) collaborated special issue about chloroplasts and mitochondria is now out in Plant and Cell Physiology with adorable cover art for it!


Zhou C, Okuno M, Nakazato I, Tsutsumi N, and Arimura S (2023) Targeted A-to-G base editing in the organellar genomes of Arabidopsis with monomeric programmable deaminases. Plant Physiol, https://doi.org/10.1093/plphys/kiad678

Arimura S and Nakazato I (2023) Genome editing of plant mitochondrial and chloroplast genomes. Plant Cell Physiol, https://doi.org/10.1093/pcp/pcad162

Dinh LT, Ueda Y, Gonzalez D, Tanaka JP, Takanashi H, and Wissuwa M (2023) Novel QTL for lateral root density and length improve phosphorus uptake in Rice (Oryza sativa L.). Rice, 16: 37.

Koji T, Iwata H, Ishimori M, Takanashi H, Yamasaki Y, Tsujimoto H (2023) Genetic dissection of seasonal changes in a greening plant based on time-series multispectral imaging. Plants,12: 3597.

Sakurai K, Toda Y, Hamazaki K, Ohmori Y, Yamasaki Y, Takahashi H, Takanashi H, Tsuda M, Tsujimoto H, Kaga A, Nakazono M, Fujiwara T, and Iwata H (2023) Random regression for modeling soybean plant response to irrigation changes using time-series multispectral data. Front Plant Sci, 14: 10.3389/fpls.2023.1201806.

Koji T, Iwata H, Ishimori M, Takanashi H, Yamasaki Y, and Tsujimoto H (2023) Multispectral phenotyping and genetic analyses of spring appearance in greening plant, Phedimus spp. Plant Phenomics, 5: 0063. DOI: 10.34133/plantphenomics.0063

Nakazato I, Okuno M, Itoh T, Tsutsumi N, and Arimura SI (2023) Characterization and development of a plastid genome base editor, ptpTALECD. Plant J, 115 (4): 1151-1162 https://doi.org/10.1111/tpj.16311


Ranaivo HN, Lam DT, Ueda Y, Tanaka JP, Takanashi H, Ramanankaja L, Razafimbelo T, and Wissuwa M (2023) QTL mapping for early root and shoot vigor of upland rice (Oryza sativa L.) under P deficient field conditions in Japan and Madagascar. Front Plant Sci, 13: 1123825.

Takanashi H (2023) Genetic control of morphological traits useful for improving sorghum. Breed Sci, 73: 57-69. https://doi.org/10.1270/jsbbs.22069

Kazama T and Arimura SI (2023) A method for precisely identifying modifications to plant mitochondrial genomes by mitoTALENs. Methods Mol Biol, 2615: 365-378. doi: 10.1007/978-1-0716-2922-2_25.

Ayabe H, Toyoda A, Iwamoto A, Tsutsumi N, and Arimura SI (2023) Mitochondrial gene defects in Arabidopsis can broadly affect mitochondrial gene expression through copy number. Plant Physiol, 191: 2256-2275. doi: 10.1093/plphys/kiad024.

中里一星, 有村慎一 (2023) シロイヌナズナの葉緑体とミトコンドリアのゲノム編集 (標的一塩基置換). Annual Report 2022 (東京大学低温科学研究センター): 28-33. http://www.crc.u-tokyo.ac.jp/publication/annualreport/parts/AnnualReport2022.pdf

中里一星, 有村慎一 (2023) 葉緑体と植物ミトコンドリアのゲノム編集. ゲノム編集の最新技術と医薬品・遺伝子治療・農業・水畜産物・有用物質生産への活用 (情報技術協会): 241-246.

髙梨秀樹, 岩田洋佳, 堤伸浩 (2023) ゲノミックセレクションを活用した草本バイオマスの増産. アグリバイオ, 2023-06: 23-27. 

津田麻衣, 髙橋宏和, 髙梨秀樹 (2023) 植物と微生物叢の相互作用を取り入れた育種戦略とその挑戦. アグリバイオ, 2023-03: 33-36. 

有村慎一(2023)ミトコンドリアと葉緑体のゲノム編集 二重膜共生オルガネラのゲノム編集技術.化学と生物, 2023-12, 61:596-602.https://katosei.jsbba.or.jp/index.php?aid=1774&back_bn_show=204&bt=on


Katsuma S, Hirota K, Matsuda-Imai N, Fukui T, Muro T, Nishino K, Kosako H, Shoji K, Takanashi H, Fujii T, Arimura SI, and Kiuchi T (2022) A Wolbachia factor for male killing in lepidopteran insects. Nat Commun, 13: 6764-6764.

Kumaishi K, Usui E, Suzuki K, Kobori S, Sato T, Toda Y, Takanashi H, Shinozaki S, Noda M, Takakura A, Matsumoto K, Yamasaki Y, Tsujimoto H, Iwata H, and Ichihashi Y (2022) High throughput method of 16S rRNA gene sequencing library preparation for plant root microbial community profiling. Sci Rep, 12: 19289-19289.

Takanashi H, Kajiya-Kanegae H, Nishimura A, Yamada J, Ishimori M, Kobayashi M, Yano K, Iwata H, Tsutsumi N, and Sakamoto W (2022) DOMINANT AWN INHIBITOR encodes the ALOG protein originating from gene duplication and inhibits awn elongation by suppressing cell proliferation and elongation in sorghum. Plant Cell Physiol, 10.1903/pcp/pcac057. ※プレスリリースはこちら.

Wacera FW, Yamazaki K, Jing ZH, Takami T, Kamiya T, Kajiya-Kanegae H, Takanashi H, Iwata H, Tsutsumi N, Fujiwara T, and Sakamoto W (2022) Sorghum ionomics reveals the functional SbHMA3a allele that limits excess Cadmium accumulation in grains. Plant Cell Physiol, 63: 713–728.

Nakazato I, Okuno M, Zhou C, Itoh T, Tsutsumi N, Takenaka M, and Arimura SI (2022) Targeted base editing in the mitochondrial genome of Arabidopsis thaliana. Proc Natl Acad Sci U S A, 10.1073/pnas.2121177119.


Sakurai K, Toda Y, Kajiya-Kanegae H, Ohmori Y, Yamasaki Y, Takahashi H, Takanashi H, Tsuda M, Tsujimoto H,  Kaga A, Nakazono M, Fujiwara T, and Iwata H (2022) Time-series multispectral imaging in soybean for improving biomass and genomic prediction accuracy. Plant Genome, e20244.  

Toda Y, Sasaki G, Ohmori Y, Yamasaki Y, Takahashi H, Takanashi H, Tsuda M, Kajiya-Kanegae H, Lopez-Lozano R, Tsujimoto H, Kaga A, Nakazono M, Fujiwara T, Baret F, and Iwata H (2022) Genomic Prediction of Green Fraction Dynamics in Soybean Using Unmanned Aerial Vehicles Observations. Front Plant Sci, 13| https://doi.org/10.3389/fpls.2022.828864.

Kuwabara K, Arimura SI, Shirasawa K, and Ariizumi T (2022) orf137 triggers cytoplasmic male sterility in tomato. Plant Physiol, kiac082, https://doi.org/10.1093/plphys/kiac082.

Takatsuka A, Kazama T, Arimura SI, and Toriyama, K (2022) TALEN-mediated depletion of the mitochondrial gene orf312 proves that it is a Tadukan-type cytoplasmic male sterility-causative gene in rice. Plant J, DOI10.1111/tpj.15715.

Kang BH, Anderson CT, Arimura SI, Bayer E, Bezanilla M, Botella MA, Brandizzi F, Burch-Smith TM, Chapman KD, Dünser K, Gu Y, Jaillais Y, Kirchhoff H, Otegui MS, Rosado A, Tang Y, Kleine-Vehn J, Wang P, and Zolman BK (2022) A glossary of plant cell structures: Current insights and future questions. Plant Cell, 34: 10-52.

中里一星, 有村慎一 (2022) 葉緑体と植物ミトコンドリアのゲノム編集. アグリバイオ, 6 (9): 38-40

中里一星, 有村慎一 (2022) シロイヌナズナの葉緑体ゲノムの標的一塩基置換. 光合成研究, 32 (1): 12-17

中里一星, 有村慎一 (2022) 植物オルガネラゲノム編集法. ひとりではじめる植物バイオテクノロジー入門 (国際文献社): 387-395

中里一星, 有村慎一 (2022) 葉緑体ゲノムの標的一塩基置換. バイオサイエンスとインダストリー, 80: 36-37.


Arimura SI (2021) MitoTALENs: A Method for Targeted Gene Disruption in Plant Mitochondrial Genomes. Methods Mol Biol, 2363: 335-340.

Jing ZH, Wacera WF, Takami T, Takanashi H, Fukada F, Kawano Y, Kajiya-Kanegae H, Iwata H, Tsutsumi N, and Sakamoto W (2021) NB-LRR-encoding genes conferring susceptibility to organophosphate pesticides in sorghum. Sci Rep, 11: 19828.

Ishimori M, Takanashi H, Fujimoto M, Kajiya-Kanegae H, Yoneda J, Tokunaga T, Tsutsumi N, and Iwata H (2021) Spatial kernel models capturing field heterogeneity for accurate estimation of genetic potential. Breed Sci, 71: 444-455.

Omukai S, Arimura SI, Toriyama K, and Kazama T (2021) Disruption of mitochondrial open reading frame 352 partially restores pollen development in cytoplasmic male sterile rice. Plant Physiol, 187: 236-246.

Ishimori M, Takanashi H, Fukami K, Cervantes K, Nagano AJ, Kajiya-Kanegae H, Grauke LJ, Tsutsumi N, Randall J, and Iwata H (2021) Genome-wide RAD-Seq analysis revealed subpopulation structures of the pecan (Carya illinoinensis) germplasm collection and their relationship to geographical distribution patterns. Acta Hortic, 1318: 177-183.

Fukushima S, Akita K, Takagi T, Kobayashi K, Moritoki N, Sugaya H, Arimura SI, Kuroiwa H, Kuroiwa T, and Nagata N (2021) Existence of giant mitochondria-containing sheet structures lacking cristae and matrix in the etiolated cotyledon of Arabidopsis thaliana. Protoplasma, https://doi.org/10.1007/s00709-021-01696-0.

Masutani B, Arimura SI, Morishita S (2021) Investigating the mitochondrial genomic landscape of Arabidopsis thaliana by long-read sequencing. PLoS Comput Biol, 17: e1008597.

Nakazato I, Okuno M, Yamamoto H, Tamura Y, Itoh T, Shikanai T, Takanashi H, Tsutsumi N, and Arimura SI (2021) Targeted base editing in the plastid genome of Arabidopsis thaliana. Nat Plants, 7: 906-913.


Takanashi H*,Shichijo M, Sakamoto L, Kajiya-Kanegae H, Iwata H, Sakamoto W and Tsutsumi N* (2021) Genetic dissection of QTLs associated with spikelet-related traits and grain size in sorghum. Sci Rep, 11: 9398. (*Co-corresponding authors)


Ayabe H, Kawai N, Shibamura M, Fukao Y, Fujimoto M, Tsutsumi N, and Arimura S (2021) FMT, a protein that affects mitochondrial distribution, interacts with translation-related proteins in Arabidopsis thaliana. Plant Cell Rep, 40: 327-337.


Ishimori M, Takanashi H, Hamazaki K, Atagi Y, Kajiya-Kanegae H, Fujimoto M, Yoneda J, Tokunaga T, Tsutsumi N and Iwata H (2020) Dissecting the Genetic Architecture of Biofuel-Related Traits in a Sorghum Breeding Population. G3-GENES GENOMES GENETICS, 10: 4565-4577. 

Ishimori M, Hattori T, Yamazaki K, Takanashi H, Fujimoto M, Kajiya-Kanegae H, Yoneda J, Tokunaga T, Fujiwara T, Tsutsumi N and Iwata H (2020) Impacts of dominance effects on genomic prediction of sorghum hybrid performance. Breed. Sci, 70: 605-616.

Arimura SI, Ayabe H, Sugaya H, Okuno M, Tamura Y, Tsuruta Y, Watari Y, Yanase S, Yamauchi T, Itoh T, Toyoda A, Takanashi H and Tsutsumi N (2020) Targeted gene disruption of ATP synthases 6-1 and 6-2 in the mitochondrial genome of Arabidopsis thaliana by mitoTALENs. Plant J, 104: 1459-1471.

Fujimoto M, Ebine K, Nishimura K, Tsutsumi N and Ueda T (2020) Longin R-SNARE is retrieved from the plasma membrane by ANTH domain-containing proteins in Arabidopsis. Proc Natl Acad Sci U S A, 117: 25150-25158.

Yamauchi T, Nakazono M, Inukai Y and Tsutsumi N (2020) Distance-to-Time Conversion Using Gompertz Model Reveals Age-Dependent Aerenchyma Formation in Rice Roots. Plant Physiol, 183: 1424-1427.

Kajiya-Kanegae H*, Takanashi H*, Fujimoto M, Ishimori M, Ohnishi N, Wacera WF, Omollo EA, Kobayashi M, Yano K, Nakano M, Kozuka T, Kusaba M, Iwata H, Tsutsumi N** and Sakamoto W** (2020) RAD-seq-Based High-Density Linkage Map Construction and QTL Mapping of Biomass-Related Traits in Sorghum using the Japanese Landrace Takakibi NOG. Plant Cell Physiol, 61: 1262–1272. (*Co-first authors, **Co-corresponding authors)

Yamauchi T, Tanaka A, Tsutsumi N, Inukai Y and Nakazono M (2020) A Role for Auxin in Ethylene-Dependent Inducible Aerenchyma Formation in Rice Roots. PLANTS-BASEL, 9: 610.

Yamazaki K, Ishimori M, Kajiya-Kanegae H, Takanashi H, Fujimoto M, Yoneda J, Yano K, Koshiba T, Tanaka R, Iwata H, Tokunaga T, Tsutsumi Nand Fujiwara T (2020) Effect of salt tolerance on biomass production in a large population of sorghum accessions. Breed Sci, 70: 167-175.


Sakamoto L, Kajiya-Kanegae H, Noshita K, Takanashi H, Kobayashi M, Kudo T, Yano K, Tokunaga T, Tsutsumi Nand Iwata H (2019) Comparison of shape quantification methods for genomic prediction, and genome-wide association study of sorghum seed morphology. PLOS One, 14: e0224695.

Li F, Shimizu A, Nishio T, Tsutsumi N and Kato H (2019) Comparison and Characterization of Mutations Induced by Gamma-Ray and Carbon-Ion Irradiation in Rice (Oryza sativa L.) Using Whole-Genome Resequencing. G3-GENES GENOMES GENETICS, 9: 3743-3751.

Yamauchi T, Tanaka A, Inahashi H, Nishizawa NK, Tsutsumi N, Inukai Y and Nakazono M (2019) Fine control of aerenchyma and lateral root development through AUX/IAA- and ARF-dependent auxin signaling. Proc Natl Acad Sci U S A, 116: 20770-20775.

Kazama T*, Okuno M, Watari Y, Yanase S, Koizuka C, Tsuruta Y, Sugaya H, Toyoda A, Itoh T, Tsutsumi N, Toriyama K, Koizuka N* and Arimura SI* (2019) Curing cytoplasmic male sterility via TALEN-mediated mitochondrial genome editing. Nat Plants, 5: 722-730. (*Co-corresponding authors)


Yamauchi T, Abe F, Tsutsumi N and Nakazono M (2019) Root Cortex Provides a Venue for Gas-Space Formation and Is Essential for Plant Adaptation to Waterlogging. Front Plant Sci, 10: 259.


Fujimoto M, Sazuka T, Oda Y, Kawahigashi H, Wu J, Takanashi H, Ohnishi T, Yoneda J, Ishimori M, Kajiya-Kanegae H, Hibara K, Ishizuna F, Ebine K, Ueda T, TokunagaT, Iwata H, Matsumoto T, Kasuga S, Yonemaru J* and Tsutsumi N* (2018) Transcriptional switch for programmed cell death in pith parenchyma of sorghum stems. Proc Natl Acad Sci U S A, doi: 10.1073/pnas.1807501115. (*Co-corresponding authors)


Takanashi H, Sumiyoshi H, Mogi M, Hayashi Y, Ohnishi T and Tsutsumi N (2018) mRNAs control HAM1 functions at the single-cell-layer level and are essential for normal embryogenesis in Arabidopsis. Plant Mol Biol, 96: 627-640.

Arimura SI, Yanase S, Tsutsumi N and Koizuka N (2018) The mitochondrial genome of an asymmetrically cell-fused rapeseed, Brassica napus, containing a radish-derived cytoplasmic male sterility-associated gene. Genes Genet Syst, 93: 143-148.

Shimano S, Hibara KI, Furuya T, Arimura SI, Tsukaya H and Itoh JI (2018) Conserved functional control, but distinct regulation, of cell proliferation in rice and Arabidopsis leaves revealed by comparative analysis of GRF-INTERACTING FACTOR 1 orthologs. Development, 145: dev159624.

Shibata S, Arimura SI, Ishikawa T and Awai K (2018) Alterations of Membrane Lipid Content Correlated With Chloroplast and Mitochondria Development in Euglena gracilis. Front Plant Sci, 9: 370.

Arimura SI (2018) Fission and Fusion of Plant Mitochondria, and Genome Maintenance. Plant Physiol, 176: 152–161.


Arimura SI, Kurisu R, Sugaya H, Kadoya N, and Tsutsumi N (2017) Cold Treatment Induces Transient Mitochondrial Fragmentation in Arabidopsis thaliana in a Way that Requires DRP3A but not ELM1 or an ELM1-Like Homologue, ELM2. Int J Mol Sci, 18: E2161.

Nagaoka N*, Yamashita A*, Kurisu R, Watari Y, Ishizuna F, Tsutsumi N, Ishizaki K, Kohchi T, and Arimura SI (2017) DRP3 and ELM1 are required for mitochondrial fission in the liverwort Marchantia polymorpha. Sci Rep, 7: 4600. (*Co-first authors)

※Scientific reports日本語版「おすすめのコンテンツ」に選んでいただきました.

Kobayashi M, Ohyanagi H, Takanashi H, Asano S, Kudo T, Kanegae H, Nagano AJ, Tainaka H, Tokunaga T, Sazuka T, Iwata H, Tsutsumi N, and Yano K (2017) Heap: a highly sensitive and accurate SNP detection tool for low coverage high-throughput sequencing data. DNA Res, 24: 397-405.

Yamauchi T, Yoshioka M, Fukazawa A, Mori H, Nishizawa NK, Tsutsumi N, Yoshioka H, and Nakazono M (2017) An NADPH Oxidase RBOH Functions in Rice Roots during Lysigenous Aerenchyma Formation under Oxygen-Deficient Conditions. Plant Cell, 29: 775-790.

Watanabe K, Guo W, Arai K, Takanashi H, Kajiya-Kanegae H, Kobayashi M, Yano K, Tokunaga T, Fujiwara T, Tsutsumi N, and  Iwata H (2017) High-Throughput Phenotyping of Sorghum Plant Height Using an Unmanned Aerial Vehicle and Its Application to Genomic Prediction Modeling. Front Plant Sci, 8: 421.

Ito Y, Toyooka K, Fujimoto M, Ueda T, Uemura T, and Nakano A (2017) The trans-Golgi network and the Golgi stacks behave independently during regeneration after Brefeldin A treatment in tobacco BY-2 cells. Plant Cell Physiol, 58: 811-821.

Kazama T, Nishimura A, and Arimura SI (2017) Chapter 4. Rice organelle genomics, approaches to genetic engineering and breeding. Takuji Sasaki and Motoyuki Ashikari edit. Rice Genomics, Genetics and Breeding. Springer Nature. 

有村 慎一, 高梨 秀樹 (2017) 植物ミトコンドリアゲノムの不思議とその改変の試み. 植物の生長調節, 52(1): 25-30.


Kokusho R, Koh Y, Fujimoto M, Shimada T, and  Katsuma S (2016) Bombyx mori nucleopolyhedrovirus BM5 protein regulates progeny virus production and viral gene expression. Virology, 498: 240-249.

Yoshinari A, Fujimoto M, Ueda T, Inada N, Naito S, and Takano J (2016) DRP1-Dependent Endocytosis is Essential for Polar Localization and Boron-Induced Degradation of the Borate Transporter BOR1 in Arabidopsis thaliana. Plant Cell Physiol, 57: 1985-2000.

Sugano Y, Kokusho R, Ueda M, Fujimoto M, Tsutsumi N, Shimada T, Kiuchi T, and Katsuma S (2016) Identification of a bipartite nuclear localization signal in the silkworm Masc protein. FEBS Lett, 590: 2256-61.

Ueda M, Tsutsumi N, and Fujimoto M (2016) Salt stress induces internalization of plasma membrane aquaporin into the vacuole in Arabidopsis thaliana. Biochem Biophys Res Commun, 474: 742-746.

Yamashita A, Fujimoto M, Katayama K, Tsutsumi N, and Arimura SI (2016) Mitochondrial outer membrane forms bridge between two mitochondria in Arabidopsis thaliana. Plant Signal Behav, 11: e1167301.

Yamashita A, Fujimoto M, Katayama K, Yamaoka S, Tsutsumi N, and Arimura SI (2016) Formation of Mitochondrial Outer Membrane Derived Protrusions and Vesicles in Arabidopsis thaliana. PLoS ONE, 11: e0146717. 

Arimura SI and Tsutsumi N (2016) Mitochondrial and peroxisomal division. Molecular Cell Biology of the Growth and Differentiation of Plant Cells, Edited by Ray J. Rose, 51–65 CRC press, ISBN: 978-1-4987-2602-3, DOI: 10.1201/b20316-6


Kanazawa T, Era A, Minamino N, Shikano Y, Fujimoto M, Uemura T, Nishihama R, Yamato KT, Ishizaki K, Nishiyama T, Kohchi T, Nakano A, and Ueda T (2015) SNARE Molecules in Marchantia polymorpha: Unique and Conserved Features of the Membrane Fusion Machinery. Plant Cell Physiol, 57: 307–324.

Baral A, Irani NG, Fujimoto M, Nakano A, Mayor S, and Mathew MK (2015) Salt-induced remodeling of spatially restricted clathrin-independent endocytic pathways in Arabidopsis root. Plant Cell, 27: 1297-315.

Yamauchi T, Shiono K, Nagano M, Fukazawa A, Ando M, Takamure I, Mori H, Nishizawa NK, Kawai-Yamada M, Tsutsumi N, Kato K, and Nakazono M (2015) Ethylene Biosynthesis Is Promoted by Very-Long-Chain Fatty Acids during Lysigenous Aerenchyma Formation in Rice Roots. Plant Physiol, 169: 180-193.


Fujimoto M, Suda Y, Vernhettes S, Nakano A and Ueda T (2014) Phosphatidylinositol 3-kinase and 4-kinase have distinct roles in intracellular trafficking of cellulose synthase complex in Arabidopsis thaliana. Plant Cell Physiol, 56: 287-298.

Huang J, Fujimoto M, Fujiwara M, Fukao Y, Arimura S and Tsutsumi N (2014) Arabidopsis dynamin-related proteins, DRP2A and DRP2B, function coordinately in post-Golgi trafficking. Biochem Biophys Res Commun, 456: 238-244.

Fujimoto M and Tsutsumi N (2014) Dynamin-related proteins in plant post-Golgi traffic. Front Plant Sci, 5: 408.

Shiono K, Ando M, Nishiuchi S, Takahashi H, Watanabe K, Nakamura M, Matsuo Y, Yasuno N, Yamanouchi U, Fujimoto M, Takanashi H, Ranathunge K, Franke RB, Shitan N, Nishizawa NK, Takamure I, Yano M, Tsutsumi N, Schreiber L, Yazaki K, Nakazono M and Kato K (2014) RCN1/OsABCG5, an ATP-binding cassette (ABC) transporter, is required for hypodermal suberization of roots in rice (Oryza sativa). Plant J, 80: 40-51.

Shiono K, Yamauchi T, Yamazaki S, Mohanty B, Malik AI, Nagamura Y, Nishizawa NK, Tsutsumi N, Colmer TD and Nakazono M (2014) Microarray analysis of laser-microdissected tissues indicates the biosynthesis of suberin in the outer part of roots during formation of a barrier to radial oxygen loss in rice (Oryza sativa). J Exp Bot, 65: 4795-4806.

Takahashi H, Greenway H, Matsumura H, Tsutsumi N and Nakazono M (2014) Rice alcohol dehydrogenase 1 promotes survival and has a major impact on carbohydrate metabolism in the embryo and endosperm when seeds are germinated in partially oxygenated water. Ann Bot, 113: 851-859.

Hu Z, Yamauchi T, Yang J, Jikumaru Y, Tsuchida-Mayama T, Ichikawa H, Takamure I, Nagamura Y, Tsutsumi N, Yamaguchi S, Kyozuka J and Nakazono M (2014) Strigolactone and cytokinin act antagonistically in regulating rice mesocotyl elongation in darkness. Plant Cell Physiol, 55: 30-41.


Matsuo Y, Arimura S, and Tsutsumi N (2013) Distribution of cellulosic wall in the anthers of Arabidopsis during microsporogenesis. Plant Cell Rep, 32: 1743-1750.

Kubo T, Fujita M, Takahashi H, Nakazono M, Tsutsumi N and Kurata N (2013) Transcriptome Analysis of Developing Ovules in Rice Isolated by Laser Microdissection. Plant Cell Physiol, 54: 750-765.

Asaoka R, Uemura T, Ito J, Fujimoto M, Ito E, Ueda T, and Nakano A (2013) Arabidopsis RABA1 GTPases are involved in transport between the trans-Golgi network and the plasma membrane, and are required for salinity stress tolerance. Plant J, 73: 240-249.


Shoji K, Kiuchi T, Hara K, Kawamoto M, Kawaoka S, Arimura S, Tsutsumi N, Sugano S, Suzuki Y, and Shimada T (2012) Characterization of a novel chromodomain-containing gene from the silkworm, Bombyx mori. GENE, 527: 649-654.

Nagano M, Takahara K, Fujimoto M, Tsutsumi N, Uchimiya H and Kawai-Yamada M (2012) Arabidopsis Sphingolipid Fatty Acid 2-Hydroxylases (AtFAH1 and AtFAH2) Are Functionally Differentiated in Fatty Acid 2-Hydroxylation and Stress Responses. Plant Physiol, 159: 1138-1148.

Wang F, Liu P, Zhang Q, Zhu J, Chen T, Arimura S, Tsutsumi N and Lin J (2012) Phosphorylation and ubiquitination of dynamin-related proteins (AtDRP3A/3B) synergically regulate mitochondrial proliferation during mitosis. Plant J, 72: 43-56.

Ito Y, Uemura T, Shoda K, Fujimoto M, Ueda T, and Nakano A (2012) cis-Golgi proteins accumulate near the ER exit sites and act as the scaffold for Golgi regeneration after brefeldin A treatment in tobacco BY-2 cells. Mol Biol Cell, 23: 3203-3214.

Ito E, Fujimoto M, Ebine K, Uemura T, Ueda T, and Nakano A (2012) Dynamic behavior of clathrin in Arabidopsis thaliana unveiled by live imaging. Plant J, 69: 204-216.

Hachiya T, Watanabe CK, Fujimoto M, Ishikawa T, Takahara K, Kawai-Yamada M, Uchimiya H, Uesono Y, Terashima I, and Noguchi K (2012) Nitrate Addition Alleviates Ammonium Toxicity Without Lessening Ammonium Accumulation, Organic Acid Depletion and Inorganic Cation Depletion in Arabidopsis thaliana Shoots. Plant Cell Physiol, 53: 577-591.

Fujimoto M, and Ueda T (2012) Conserved and plant-unique mechanisms regulating plant post-Golgi traffic. Front Plant Sci, 3: 197.


Aya K, Suzuki G, Suwabe K, Hobo T, Takahashi H, Shiono K, Yano K, Tsutsumi N, Nakazono M, Nagamura Y, Matsuoka M and Watanabe M (2011) Comprehensive network analysis of anther-expressed genes in rice by the combination of 33 laser microdissection and 143 spatiotemporal microarrays. PLoS One, 6: e26162.

Takahashi H, Saika H, Matsumura H, Nagamura Y, Tsutsumi N, Nishizawa NK and Nakazono M (2011) Cell division and cell elongation in the coleoptile of rice alcohol dehydrogenase 1-deficient mutant are reduced under complete submergence. Ann Bot, 108: 253-261.

Tanabe Y, Maruyama JI, Yamaoka S, Yahagi D, Matsuo I, Tsutsumi N and Kitamoto K (2011) Peroxisomes are involved in biotin biosynthesis in Aspergillus and Arabidopsis. J Biol Chem, 286: 30455-30461.

Takanashi H, Ohnishi T, Mogi M, Hirata Y and Tsutsumi N (2011) DCL2 is highly expressed in the egg cell in both rice and Arabidopsis. Plant Signal Behav, 6: 604-606.

Ebine K, Fujimoto M, Okatani Y, Nishiyama T, Goh T, Ito E, Dainobu T, Nishitani A, Uemura T, Sato MH, Thordal-Christensen H, Tsutsumi N, Nakano A and Ueda T (2011) A membrane trafficking pathway regulated by the plant-specific RAB GTPase ARA6. Nat Cell Biol, 13: 853-859. 

Bashir K, Ishimaru Y, Shimo H, Nagasaka S, Fujimoto M, Takanashi H, Tsutsumi N, An G, Nakanishi H and Nishizawa NK (2011) The rice mitochondrial iron transporter is essential for plant growth. Nat Commun, 2: 322.

Ohnishi T*, Takanashi H*, Mogi M, Takahashi H, Kikuchi S, Yano K, Okamoto T, Fujita M, Kurata N and Tsutsumi N (2011) Distinct gene expression profiles in egg and synergid cells of rice as revealed by cell type-specific microarrays. Plant Physiol, 155: 881-891. (* Co-first authors)

Yamaoka S, Nakajima M, Fujimoto M and Tsutsumi N (2011) MIRO1 influences the morphology and intracellular distribution of mitochondria during embryonic cell division in Arabidopsis. Plant Cell Rep, 30: 239-244.

Rajhi I*, Yamauchi T*, Takahashi H, Nishiuchi S, Shiono K, Watanabe R, Mliki A, Nagamura Y, Tsutsumi N, Nishizawa NK and Nakazono M (2011) Identification of genes expressed in maize root cortical cells during lysigenous aerenchyma formation using laser microdissection and microarray analyses. New Phytol, 190: 351-368. (* Co-first authors)


Roy S, Ueda M, Kadowaki K and Tsutsumi N (2010) Different status of the gene for ribosomal protein S16 in the chloroplast genome during evolution of the genus Arabidopsis and closely related species. Genes Genet Syst, 85: 319-326.

Zhang D, Kato Y, Zhang L, Fujimoto M, Tsutsumi N, Sodmergen and Sakamoto W (2010) The FtsH protease heterocomplex in Arabidopsis: dispensability of type-B protease activity for proper chloroplast development. Plant Cell, 22: 3710-3725.

Shiono K, Ogawa S, Yamazaki S, Isoda H, Fujimura T, Nakazono M and Colmer TD (2010) Contrasting dynamics of radial O2 loss barrier induction and aerenchyma formation in rice roots of two lengths. Ann Bot, 107: 89-99.

Wakamatsu K, Fujimoto M, Nakazono M, Arimura S and Tsutsumi N (2010) Fusion of mitochondria in tobacco suspension cultured cells is dependent on the cellular ATP level but not on actin polymerization. Plant Cell Rep, 29: 1139-1145.

Hu Z, Yan H, Yang J, Yamaguchi S, Maekawa M, Takamure I, Tsutsumi N, Kyozuka J and Nakazono M (2010) Strigolactones negatively regulate mesocotyl elongation in rice during germination and growth in darkness. Plant Cell Physiol, 51: 1136-1142.

Fujimoto M, Arimura S, Ueda T, Takanashi H, Hayashi Y, Nakano A and Tsutsumi N (2010) Arabidopsis dynamin-related proteins DRP2B and DRP1A participate together in clathrin-coated vesicle formation during endocytosis. Proc Natl Acad Sci U S A, 107: 6094-6099.

Takahashi H, Kamakura H, Sato Y, Shiono K, Abiko T, Tsutsumi N, Nagamura Y, Nishizawa NK and Nakazono M (2010) A method for obtaining high quality RNA from paraffin sections of plant tissues by laser microdissection. J Plant Res, 123: 807-813.

Doniwa Y, Ueda M, Ueta M, Wada A, Kadowaki K and Tsutsumi N (2010) The involvement of a PPR protein of the P subfamily in partial RNA editing of an Arabidopsis mitochondrial transcript. Gene, 454: 39-46.

Takanashi H, Ohnishi T, Mogi M, Okamoto T, Arimura S and Tsutsumi N (2010) Studies of mitochondrial morphology and DNA amount in the rice egg cell. Curr Genet, 56: 33-41.

Kubo N and Arimura S (2010) Discovery of the rpl10 gene in diverse plant mitochondrial genomes and its probable replacement by the nuclear gene for chloroplast RPL10 in two lineages of angiosperms. DNA Res, 17: 1-9.

有村 慎一, 山岡 尚平, 高梨 秀樹, 堤 伸浩 (2010) 高等植物ミトコンドリアのダイナミクスとその特質. 細胞工学, 29: 448-452.


Ishimaru Y, Bashir K, Fujimoto M, An G, Itai RN, Tsutsumi N, Nakanishi H and Nishizawa NK (2009) Rice-specific mitochondrial iron-regulated gene (MIR) plays an important role in iron homeostasis. Mol Plant, 2: 1059-1066.

Fujimoto M*, Arimura S*, Mano S, Kondo M, Saito C, Ueda T, Nakazono M, Nakano A, Nishimura M and Tsutsumi N (2009) Arabidopsis dynamin-related proteins DRP3A and DRP3B are functionally redundant in mitochondrial fission, but have distinct roles in peroxisomal fission. Plant J, 58: 388-400. (*Co-first authors)

Nagano M, Ihara-Ohori Y, Imai H, Inada N, Fujimoto M, Tsutsumi N, Uchimiya H and Kawai-Yamada M (2009) Functional association of cell death suppressor, Arabidopsis Bax inhibitor-1, with fatty acid 2-hydroxylation through cytochrome b. Plant J, 58: 122-134.

Inoue H, Kobayashi T, Nozoye T, Takahashi M, Kakei Y, Suzuki K, Nakazono M, Nakanishi H, Mori S and Nishizawa NK (2009) Rice OsYSL15 is an iron-regulated iron(III)-deoxymugineic acid transporter expressed in the roots and is essential for iron uptake in early growth of the seedlings. J Biol Chem, 284: 3470-3479.

Okamoto S, Ohnishi E, Sato S, Takahashi H, Nakazono M, Tabata S and Kawaguchi M (2009) Nod factor/nitrate-induced CLE genes that drive HAR1-mediated systemic regulation of nodulation. Plant Cell Physiol, 50: 67-77.


Kubo N, Fujimoto M, Arimura S, Hirai M and Tsutsumi N (2008) Transfer of rice mitochondrial ribosomal protein L6 gene to the nucleus: acquisition of the 5'-untranslated region via a transposable element. BMC Evol Bio, 8: 314.

Arimura S, Fujimoto M, Doniwa Y, Kadoya N, Nakazono M, Sakamoto W and Tsutsumi N (2008) Arabidopsis ELONGATED MITOCHONDRIA1 is required for the localization of dynamin-related protein DRP3A to mitochondrial fission sites. Plant Cell, 20: 1555-1566.

Hobo T, Suwabe K, Aya K, Suzuki G, Yano K, Ishimizu T, Fujita M, Kikuchi S, Hamada K, Miyano M, Fujioka T, Kaneko F, Kazama T, Mizuta Y, Takahashi H, Shiono K, Nakazono M, Tsutsumi N, Nagamura Y, Kurata N, Watanabe M and Matsuoka M (2008) Various spatiotemporal expression profiles of anther-expressed genes in rice. Plant Cell Physiol, 49: 1417-1428.

Suwabe K, Suzuki G, Takahashi H, Shiono K, Endo M, Yano K, Fujita M, Masuko H, Saito H, Fujioka T, Kaneko F, Kazama T, Mizuta Y, Kawagishi-Kobayashi M, Tsutsumi N, Kurata N, Nakazono M and Watanabe M (2008) Separated transcriptomes of male gametophyte and tapetum in rice: validity of a laser microdissection (LM) microarray. Plant Cell Physiol, 49: 1407-1416.

Fujimoto M, Arimura S, Nakazono M and Tsutsumi N (2008) Arabidopsis dynamin-related protein DRP2B is co-localized with DRP1A on the leading edge of the forming cell plate. Plant Cell Rep, 27:1581-1586.

Endo A, Sawada Y, Takahashi H, Okamoto M, Ikegami K, Koiwai H, Seo M, Toyomasu T, Mitsuhashi W, Shinozaki K, Nakazono M, Kamiya Y, Koshiba T and Nambara E (2008) Drought induction of Arabidopsis 9-cis-epoxycarotenoid dioxygenase occurs in vascular parenchyma cells. Plant Physiol, 147: 1984-1993.

Ueda M, Nishikawa T, Fujimoto M, Takanashi H, Arimura S, Tsutsumi N and Kadowaki K (2008) Substitution of the gene for chloroplast RPS16 was assisted by generation of a dual targeting signal. Mol Biol Evol, 25: 1566-1575.

Ueda M, Fujimoto M, Arimura S, Tsutsumi N and Kadowaki K (2008) Presence of a latent mitochondrial targeting signal in gene on mitochondrial genome. Mol Biol Evol, 25: 1791-1793.

Shiono K, Takahashi H, Colmer TD and Nakazono M (2008) Role of ethylene in acclimations to promote oxygen transport in roots of plants in waterlogged soils. Plant Sci (Ethylene Special Issue; Invited review), 175: 52-58.

Matsushima R, Hamamura Y, Higashiyama T, Arimura S, Sodmergen, Tsutsumi N and Sakamoto W (2008) Mitochondrial dynamics in plant male gametophyte visualized by fluorescent live imaging. Plant Cell Physiol, 49: 1074-1083.

Tani T, Sobajima H, Okada K, Chujo T, Arimura S, Tsutsumi N, Nishimura M, Seto H, Nojiri H and Yamane H (2008) Identification of the OsOPR7 gene encoding 12-oxophytodienoate reductase involved in the biosynthesis of jasmonic acid in rice. Planta, 227: 517-526.

辻 寛之, 中園 幹生 (2008) 環境ストレスに応答したヒストン修飾の制御. 植物のエピジェネティクス [島本 功, 飯田 滋, 角谷 徹仁 (編)] 細胞工学別冊 植物細胞工学, 24: 115-117.

塩野 克宏, 高橋 宏和, 中園 幹生 (2008) 植物は過湿土壌にどのように適応しているのか. 化学と生物, 46: 245-251.

Nakazono M and Hirai A (2008) Frequent DNA transfer among mitochondrial, plastid and nuclear genomes of rice during evolution. Rice Biology in the Genomics Era [Hirai H, Sasaki T, Sano Y and Hirano H (eds)] Springer, The Netherlands: 107-117.

Ohnishi T, Nakazono N and Tsutsumi N (2008) Abiotic stress. Rice Biology in the Genomics Era [Hirai H, Sasaki T, Sano Y and Hirano H (eds)] Springer, The Netherlands: 337-355.


Doniwa Y, Arimura S and Tsutsumi N (2007) Mitochondria use actin filaments as rails for fast translocation in Arabidopsis and tobacco cells. Plant Biotech, 24: 441-447.

Fujimoto M, Arimura S, Nakazono M and Tsutsumi N (2007) Imaging of plant dynamin-related proteins and clathrin around the plasma membrane by variable incidence angle fluorescence microscopy. Plant Biotech, 24: 449-455.

Ueda M, Fujimoto M, Arimura S, Murata J, Tsutsumi N and Kadowaki K (2007) Loss of the rpl32 gene from the chloroplast genome and subsequent acquisition of a preexisting transit peptide within the nuclear gene in Populus. Gene, 402: 51-56.

Yan H. Saika H, Maekawa M, Takamure I, Tsutsumi N, Kyozuka J and Nakazono M (2007) Rice tillering dwarf mutant dwarf3 has increased leaf longevity during darkness-induced senescence or hydrogen peroxide-induced cell death. Genes Genet Syst, 82: 361-366.

Ishimaru T, Nakazono M, Masumura T, Abiko M, San-oh Y, Nishizawa NK and Kondo M (2007) A method for obtaining high integrity RNA from developing aleurone cells and starchy endosperm in rice (Oryza sativa L.) by laser microdissection. Plant Sci, 173: 321-326.

Saika H, Okamoto M, Miyoshi K, Kushiro T, Shinoda S, Jikumaru Y, Fujimoto M, Arikawa T, Takahashi H, Ando M, Arimura S, Miyao A, Hirochika H, Kamiya Y, Tsutsumi N, Nambara E and Nakazono M (2007) Ethylene promotes submergence-induced expression of OsABA8ox1, a gene that encodes ABA 8 '-hydroxylase in rice. Plant Cell Physiol, 48: 287-298.

Ohtsu K, Takahashi H, Schnable PS and Nakazono M (2007) Cell type-specific gene expression profiling in plants by using a combination of laser microdissection and high-throughput technologies. Plant Cell Physiol, 48: 3-7.

Watanabe W, Shimada T, Matsunaga S, Kurihara D, Fukui K, Arimura S, Tsutsumi N, Isobe K and Itoh K (2007) Single-organelle tracking by two-photon conversion. Optics Exp, 15: 2490-2498.

中園 幹生, 三好 健太郎, 松永 幸大 (2007) ヒストン修飾による植物の環境応答. 化学と生物, 45: 51-57.


Ueda M, Fujimoto M, Arimura S, Tsutsumi N and Kadowaki K (2006) Evidence for transit peptide acquisition through duplication and subsequent frameshift mutation of a preexisting protein gene in rice. Mol Biol Evol, 23: 2405-2412.

Takanashi H, Arimura S, Sakamoto W and Tsutsumi N (2006) Different amounts of DNA in each mitochondrion in rice root. Genes Genet Syst, 81: 215-218.

Yoshinaga K, Fujimoto M, Arimura S, Tsutsumi N, Uchimiya H and Kawai-Yamada M (2006) The mitochondrial fission regulator DRP3B does not regulate cell death in plants. Ann Bot, 97: 1145-1149.

Ueda M, Arimura S, Yamamoto MP, Takaiwa F, Tsutsumi N and Kadowaki K (2006) Promoter shuffling at a nuclear gene for mitochondrial RPL27. Involvement of interchromosome and subsequent intrachromosome recombinations. Plant Physiol, 141: 702-710.

Meguro N, Tsuji H, Suzuki Y, Tsutsumi N, Hirai A and Nakazono M (2006) Analysis of expression of genes for mitochondrial aldehyde dehydrogenase in maize during submergence and following re-aeration. Breed Sci, 56: 365-370.

Tsuji H, Saika H, Tsutsumi N, Hirai A and Nakazono M (2006) Dynamic and reversible changes in histone H3-Lys4 methylation and H3 acetylation occurring at submergence-inducible genes in rice. Plant Cell Physiol, 47: 995-1003.

Tsuji H, Aya K, Ueguchi-Tanaka M, Shimada Y, Nakazono M, Watanabe R, Nishizawa NK, Gomi K, Shimada A, Kitano H, Ashikari M and Matsuoka M (2006) GAMYB controls different sets of genes and is differentially regulated by microRNA in aleurone cells and anthers. Plant J, 47: 427-444.

Saika H, Matsumura H, Takano T, Tsutsumi N and Nakazono M (2006) A point mutation of Adh1 gene is involved in repression of coleoptile elongation under submergence in rice. Breed Sci, 56: 69-74.

Ishimaru Y, Suzuki M, Tsukamoto T, Suzuki K, Nakazono M, Kobayashi T, Wada Y, Watanabe S, Matsuhashi S, Takahashi M, Nakanishi H, Mori S and Nishizawa NK (2006) Rice plants take up iron as an Fe3+-phytosiderophore and as Fe2+. Plant J, 45: 335-346.

Nakada M, Komatsu M, Ochiai T, Ohtsu K, Nakazono M, Nishizawa NK, Nitta K, Nishiyama R, Kameya T and Kanno A (2006) Isolation of MaDEF from Muscari armeniacum and analysis of its expression using laser microdissection. Plant Sci, 170: 143-150.

Kubo N, Arimura S, Tsutsumi N, Kadowaki K and Hirai M (2006) Isolation and characterization of the pea cytochrome c oxidase Vb gene. Genome, 49: 1481-1489.

松永 幸大, 栗原 大輔, 中園 幹生 (2006) 植物のヒストン翻訳後修飾研究の幕開け. 化学と生物, 44: 798-799.

高橋 宏和, 中園 幹生 (2006) レーザーマイクロダイセクション法. 日本作物学会紀事, 75: 583-585.

Meguro N, Tsuji H, Tsutsumi N, Nakazono N and Hirai A (2006) Involvement of aldehyde dehydrogenase in alleviation of post-anoxic injury in rice. Abiotic stress tolerance in plants --Toward the improvement of global environment and food-- [Rai AK and Takabe T (eds)] Springer, Netherland: 111-119.

Arimura S and Tsutsumi N (2006) Dynamic Mitochondria, their fission and fusion in higher plants. BY-2 book2, New Treatise, Biotechnology and Agriculture and Forestry, 58: 225-240.

藤本 優, 堤 伸浩, 有村 慎一 (2006) 共焦点レーザー顕微鏡 細胞工学別冊植物細胞工学シリーズ 22 “植物の細胞を観る実験プロトコール”. 

(中野 明彦, 福田 裕穂, 西村 幹夫 監修), 1章4節: 77-82.

有村慎一, 堤伸浩 (2006) 蛍光を使った観察法. 共焦点レーザー顕微鏡 細胞工学別冊植物細胞工学シリーズ 22 “植物の細胞を観る実験プロトコール”, 2章1節: 100-105.

有村慎一, 堤伸浩 (2006) ミトコンドリア. 共焦点レーザー顕微鏡 細胞工学別冊植物細胞工学シリーズ 22 “植物の細胞を観る実験プロトコール”, 3章4節: 163-167.


Ohtsu K, Hirano H, Tsutsumi N, Hirai A and Nakazono M (2005) Anaconda, a new class of transposon belonging to the Mu superfamily, has diversified by acquiring host genes during rice evolution. Mol Genet Genomics, 274: 606-615.

Nakada M, Komatsu M, Ochiai T, Ohtsu K, Nakazono M, Nishizawa NK, Nitta K, Nishiyama R, Kameya T and Kanno A (2005) Isolation of MaDEF from Muscari armeniacum and analysis of its expression using laser microdissection. Plant Sci, 170: 143-150.

Yoshinaga K, Arimura S, Hirata A, Niwa Y, Yun DJ, Tsutsumi N, Uchimiya H and Kawai-Yamada M (2005) Mammalian Bax initiates plant cell death through organelle destruction. Plant Cell Rep, 24: 408-417.

Yoshinaga K, Arimura S, Niwa Y, Tsutsumi N, Uchimiya H and Kawai-Yamada M (2005) Mitochondrial behavior in the early stages of ROS-stress leading to cell death in Arabidopsis thaliana. Ann Bot, 96: 337-342.

Saika H, Nakazono M, Ikeda A, Yamaguchi J, Masaki S, Kanekatsu M and Nemoto K (2005) A transposon-induced spontaneous mutation results in low β-amylase content in rice. Plant Sci, 169: 239-244.

Yoshida KT, Endo M, Nakazono M, Fukuda H, Demura T, Tsuchiya T and Watanabe M (2005) cDNA microarray analysis of gene expression changes during pollination, pollen-tube elongation, fertilization, and early embryogenesis in rice pistils. Sex Plant Reprod, 17: 269-275.

Arimura S and Tsutsumi N (2005) Plant Mitochondrial fission and fusion. Plant Biotech, 22: 415-418.

有村 慎一, 堤 伸浩 (2005) 高等植物ミトコンドリアの分裂と融合. 蛋白質核酸酵素, 50: 1875-1878.

目黒 直樹, 最相 大輔, 中園 幹生 (2005) 酸化ストレスとミトコンドリア機能. 蛋白質核酸酵素, 50: 1879-1882.


Feng X, Arimura S, Hirano H, Sakamoto W and Tsutsumi N (2004) Isolation of mutants with aberrant mitochondrial morphology from Arabidopsis thaliana. Genes Genet Syst, 79: 301-305.

Fujimoto M, Arimura S, Nakazono M and Tsutsumi N (2004) A Rice Dynamin-like Protein, OsDRP3A, Is Involved in Mitochondrial Fission. Breed Sci, 54: 367-372.

Li Y, Ohtsu K, Nemoto K, Tsutsumi N, Hirai A and Nakazono M (2004) The rice pyruvate decarboxylase 3 gene, which lacks introns, is transcribed in mature pollen. J Exp Bot, 55: 145-146.

Arimura S, Yamamoto J, Aida GP, Nakazono M and Tsutsumi N (2004) Frequent fusion and fission of plant mitochondria with unequal nucleoid distribution. Proc Natl Acad Sci USA, 101: 7805-7808.

Schnable PS, Hochholdinger F and Nakazono M (2004) Global expression profiling applied to plant development. Curr Opin Plant Biol, 7: 50-56.

Arimura S, Aida GP, Fujimoto M, Nakazono M and Tsutsumi N (2004) Arabidopsis dynamin-like protein 2a (ADL2a), like ADL2b, is involved in plant mitochondrial division. Plant Cell Physiol, 45: 236-242.

有村 慎一, 山本 純子, 堤 伸浩 (2004) Kaedeを使って植物ミトコンドリアの融合を観る. 実験医学, 22: 2674-2675.


Kubo N, Arimura S, Tsutsumi N, Hirai A and Kadowaki K (2003) Involvement of N-terminal region in mitochondrial targeting of rice RPS1O and RPS14 proteins. Plant Sci, 164: 1047-1055.

Tsuji H, Meguro N, Suzuki Y, Tsutsumi N, Hirai A and Nakazono M (2003) Induction of mitochondrial aldehyde dehydrogenase by submergence facilitates oxidation of acetaldehyde during re-aeration in rice. FEBS Lett, 546: 369-373.

Nakazono M, Qiu F, Borsuk LA and Schnable PS (2003) Laser capture microdissection, a tool for the global analysis of gene expression in specific plant cell types: Identification of genes differentially expressed in epidermal cells or vascular tissues of maize. Plant Cell, 15: 583-596.

Tsuji H, Tsutsumi N, Sasaki T, Hirai A and Nakazono M (2003) Organ-specific expressions and chromosomal locations of two mitochondrial aldehyde dehydrogenase genes from rice (Oryza sativa L.), ALDH2a and ALDH2b. Gene, 305: 195-204.


Ohtsu K, Ito Y, Saika H, Nakazono M, Tsutsumi N and Hirai A (2002) ABA-independent expression of rice alternative oxidase genes under environmental stresses. Plant Biotech, 19: 187-190.

Notsu Y, Masood S, Nishikawa T, Kubo N, Akiduki G, Nakazono M, Hirai A and Kadowaki K (2002) The complete sequence of rice (Oryza sativa L.) mitochondrial genome: frequent DNA sequence acquisition and loss during evolution of flowering plants. Mol Gen Genomics, 268: 434-445.

Arimura S and Tsutsumi N (2002) A dynamin-like protein (ADL2b), rather than FtsZ, is involved in Arabidopsis mitochondrial division. Proc Natl Acad Sci USA, 99: 5727-5731.

Saika H, Ohtsu K, Hamanaka S, Nakazono M, Tsutsumi N and Hirai A (2002) AOX1c, a novel rice gene for alternative oxidase; comparison with rice AOX1a and AOX1b. Genes Genet Syst, 77: 31-38.


Meguro N, Nakazono M, Tsutsumi N and Hirai A (2001) Decreased transcription of a gene encoding putative mitochondrial aldehyde dehydrogenase in barley (Hordeum vulgare L.) under submerged conditions. Plant Biotech, 18: 223-228.

Ohtsu K, Nakazono M, Tsutsumi N and Hirai A (2001) Characterization and expression of the genes for cytochrome c oxidase subunit VIb (COX6b) from rice and Arabidopsis thaliana. Gene, 264: 233-239.

Saisho D, Nakazono M, Tsutsumi N and Hirai A (2001) ATP synthesis inhibitors as well as respiratory inhibitors increase steady-state level of alternativeoxidase mRNA in Arabidopsis thaliana. J Plant Physiol, 158: 241-245.

Arimura S, Hirai A and Tsutsumi N (2001) Numerous and highly developed tubular projections from plastids observed in tobacco epidermal cells. Plant Sci, 160: 449-454.


Nakazono M, Tsuji H, Li Y, Saisho D, Arimura S, Tsutsumi N and Hirai A (2000) Expression of a gene encoding mitochondrial aldehyde dehydrogenase in rice increases under submerged conditions. Plant Physiol, 124: 587-598.

Li Y, Nakazono M, Tsutsumi N and Hirai A (2000) Molecular and cellular characterizations of a cDNA clone encoding a novel isozyme of aldehyde dehydrogenase from rice. Gene, 249: 67-74.

Tsuji H, Nakazono M, Saisho D, Tsutsumi N and Hirai A (2000) Transcript levels of the nuclear-encoded respiratory genes in rice decrease by oxygen deprivation: evidence for involvement of calcium in expression of the alternative oxidase 1a gene. FEBS Lett, 471: 201-204.

Nakazono M, Imamura T, Tsutsumi N, Sasaki T and Hirai A (2000) Characterization of two cDNA clones encoding rice F1F0-ATPase inhibitor proteins, both of which are targeted to mitochondria. Planta, 210: 188-194.


Arimura S, Takusagawa S, Hatano S, Nakazono M, Hirai A and Tsutsumi N (1999) A novel plant nuclear gene coding for chloroplast ribosomal protein S9 has a transit peptide related to that of rice chloroplast ribosomal protein L12. FEBS Lett, 450: 231-234.

Ohtsu K, Hamanaka S, Yamazaki K, Nakazono M and Hirai A (1999) Characterization of a cDNA encoding a novel subunit for cytochrome c oxidase (COX6b) from rice. Breed Sci, 49: 211-215.

Hamanaka S, Ohtsu K, Kadowaki K, Nakazono M and Hirai A (1999) Identification of cDNA encoding cytochrome c oxidase subunit 5c (COX5c) from rice: comparison of its expression with nuclear-encoded and mitochondrial-encoded COX genes. Genes Genet Syst, 74: 71-75.

Kubo N, Harada K, Hirai A and Kadowaki K (1999) A single nuclear transcript encoding mitochondrial RPS14 and SDHB of rice is processed by alternative splicing: Common use of the same mitochondrial targeting signal for different proteins. Proc Natl Acad Sci USA, 96: 9207-9211.

Watanabe A, Nakazono M, Tsutsumi N and Hirai A (1999) AtUCP2: a Novel isoform of the mitochondrial uncoupling protein of Arabidopsis thaliana. Plant Cell Physiol, 40: 1160-1166.


Kusaka M, Kurashige M, Hirai A and Tsutsumi N (1998) Characterization of two rice genes for nuclear-encoded chloroplast ribosomal protein L12 and phylogenetic analysis of the acquisition of transit peptides and gene duplication. Theor Appl Genet, 97: 110-115.

Kanazawa A, Watanabe S, Nakamoto T, Tsutsumi N and Hirai A (1998) Phylogenetic relationships in the genus Nelumbo based on polymorphism and quantitative variations in mitochondrial DNA. Genes Genet Syst, 73: 39-44.

Arimura S, Takusagawa S, Hirai A and Tsutsumi N (1998) Genomic structures and the 5'-flanking regions of the nuclear genes for chloroplast ribosomal proteins L13 and L24 of rice (Oryza sativa L.). Breed Sci, 48: 145- 149.

Kanazawa A, Tsutsumi N and Hirai A (1998) Differential changes in copy numbers of rice mitochondrial plasmid-like DNAs and main mitochondrial genomic DNAs that depend on temperature. Curr Genet, 33: 437- 444.

Miyata S, Nakazono M and Hirai A (1998) Transcription of plastid-derived tRNA genes in rice mitochondria. Curr Genet, 34: 216-220.


Saisho D, Nambara E, Naito S, Tsutsumi N, Hirai A and Nakazono M (1997) Characterization of the gene family for alternative oxidase from Arabidopsis thaliana. Plant Mol Biol, 35: 585-596.

Ito Y, Saisho D, Nakazono M, Tsutsumi N and Hirai A (1997) Transcript levels of tandem-arranged alternative oxidase genes in rice are increased by low temperature. Gene, 203: 121-129.

Miyata S and Hirai A (1997) Four kinds of plasmid-like DNA of rice mitochondria are transcribed. Breed Sci, 47: 51-55.

Kanno A, Nakazono M, Hirai A and Kameya T (1997) Chloroplast derived trnH gene is expressed in the mitochondrial genome of gramineous plants. Plant Mol Biol, 34: 353-356.

Kanno A, Nakazono M, Hirai A and Kameya T (1997) Maintenance of chloroplast-derived sequences in the mitochondrial DNA of Gramineae. Curr Genet, 32: 413-419.


Niwa T, Kanno A, Tsutsumi N and Hirai A (1996) Detailed mapping of the chloroplast genome of barley, Hordeum vulgare L. Genes Genet Syst, 71: 175-180.

Suzuki T, Kawano S, Hirai A and Kuroiwa T (1996) Variability of mitochondrial subgenomic molecules in the meristematic cells of higher plants. Genes Genet Syst, 71: 329-333.

Tsutsumi N, Takusagawa S, Suzuki H and Hirai A (1996) Molecular cloning and nucleotide sequencing of nuclear genes coding for the chloroplast ribosomal proteins L13, L24, L28 of rice (Oryza sativaL.). Plant Sci, 15: 6652-6661. 

Nakazono M, Ito Y, Tsutsumi N and Hirai A (1996) The gene for a subunit of the ABC-type heme transporter is transcribed together with the gene for subunit 6 of NADH dehydrogenase in rice mitochondria. Curr Genet, 29: 412-416.

Nakazono M, Ishikawa M, Yoshida KT, Tsutsumi N and Hirai A (1996) Multiple initiation sites for transcription of a gene for subunit 1 of F1-ATPase (atp1) in rice mitochondria. Curr Genet, 29: 417-422.

Zeltz P, Kadowaki K, Kubo N, Maier RM, Hirai A and Kossel H (1996) A promiscuous chloroplast DNA fragment is transcribed in plant mitochondria but the encoded RNA is not edited. Plant Mol Biol, 31: 647-656.

Ito Y, Nakazono M, Kadowaki K, Tsutsumi N and Hirai A (1996) RNA editing of transcripts of the gene for apocytochrome b (cob) in rice mitochondria. Genes Genet Syst, 71: 85-89.

Nakazono M, Nishiwaki S, Tsutsumi N and Hirai A (1996) A chloroplast-derived sequence is utilized as a source of promoter sequences for the gene for subunit 9 of NADH dehydrogenase (nad9) in rice mitochondria. Mol Gen Genet, 252: 371-378.

Kadowaki K, Kubo N, Ozawa K and Hirai A (1996) Targeting presequence acquisition after mitochondrial gene transfer to the nucleus occurs by duplication of existing targeting signal. EMBO J, 15: 6652-6661.